Effect of Gingival Mesenchymal Stem Cell-Derived Small Extracellular Vesicles in Management of Infra-bony Periodontal Defect

The goal of the study to evaluate whether the adjunctive use of gingival mesenchymal stem cell-derived extracellular vesicles (GMSC-derived EVs) combined with β-tricalcium phosphate (β-TCP) and a collagen membrane results in superior radiographic bone fill compared to β-TCP and collagen membrane alone in the surgical management of infra-bony periodontal defects over a 6-month follow-up period.

Inclusion Criteria * Healthy Individuals * Non-smokers * Presence of interproximal defect in the bone wall of two or three osseous estimated by periapical radiographic evaluation and probing depth ≥6 mm after S1 and S2 therapy * Loss of clinical attachment ≥4 mm after S1 and S2 treatment * Full mouth plaque score and bleeding on probing score ≤20% after S1 and S2 therapy * Only vital teeth involved * Thick gingival biotype more than 1 mm with enough width of attached gingiva. * Exclusion Criteria: * Pregnant and lactating females. * Non-cooperating patients. Individuals with decision problems (prisoners, disabled, and mentally retarded patients)