Daily Upgrade Trial

This study evaluates the acute and chronic effects of an essential amino acid supplement (Daily Upgrade EAA+) on metabolomics, amino acid kinetics, metabolic rate, body composition in active adult women. The study consists of an acute randomized double-blind crossover phase comparing EAA+, whey protein isolate, and placebo before high-intensity interval training (HIIT), and a chronic randomized double-blind parallel-arm phase evaluating 3 weeks of EAA+ or placebo supplementation combined with twice-weekly HIIT.

Inclusion Criteria: * Participant is an adult between the ages of 25-45 years * Participant has a recorded BMI between 18.5-30 kg/m\^2 * Participant is apparently healthy and free from disease, as determined by a health history questionnaire * Participant is a biological female * Participant is naturally menstruating or uses a form of hormonal contraceptive * Participant is recreationally active, based on the International Physical Activity Questionnaire- Short Form * Participant agrees to abstain from caffeine (12 hours), tobacco, alcohol, and exercise for 24 hours prior to testing days * Participant is willing and able to comply with the protocol Exclusion Criteria: * Participant has been diagnosed with chronic heart disease, arrhythmias, thyroid disease, renal, hepatic, autoimmune or neurological diseases, as determined by a health history questionnaire. * Participant has a musculoskeletal disorder or injury that would prohibit them from being able to safely complete the study protocol. * Participant is currently enrolled in a separate clinical trial or weight loss program involving diet, exercise, physical activity, or the potential ingestion of an active drug or dietary supplement * Participant is using, or has consistently used one of the following dietary supplements within 1 month prior to enrollment: whey protein, creatine, essential amino acids * Participant has a known allergy or sensitivity to the placebo or active ingredients * Participant is pregnant or planning to become pregnant * Participant does not speak English or is hearing impaired