Comparison of Growth Factor Releases and Degradation Rates of Membranes Obtained From A-PRF+, L-PRF, and Alb-PRF

Platelet-rich fibrin (PRF) is widely used in regenerative dentistry because of its autologous growth factor release and fibrin scaffold properties. Different PRF preparations may exhibit different biological characteristics that influence their clinical performance. This study aims to compare Albumin-PRF (Alb-PRF), Advanced Platelet-Rich Fibrin+ (A-PRF+), and Leukocyte-Platelet Rich Fibrin (L-PRF) regarding cumulative growth factor release and fibrin degradation. Blood samples obtained from healthy volunteers will be processed to prepare all three PRF types. Growth factor release and fibrin degradation will be analyzed under standardized laboratory conditions.

Inclusion Criteria: * Healthy volunteers aged 18 to 40 years * Male or female * Able and willing to provide written informed consent * Non-smokers * No history of systemic disease * No regular medication use * No use of antibiotics, corticosteroids, or non-steroidal anti-inflammatory drugs within the previous 6 months Exclusion Criteria: * Age younger than 18 years or older than 40 years * Smoking * Pregnancy or breastfeeding * History of any systemic disease * Regular medication use * Use of antibiotics, corticosteroids, or non-steroidal anti-inflammatory drugs within the previous 6 months * Refusal or inability to provide written informed consent