15-Hydroxyprostaglandin Dehydrogenase in Osteoarthritis and Ankylosing Spondylitis

To evaluate serum and synovial fluid 15-PGDH levels in patients with osteoarthritis and ankylosing spondylitis and determine their associations with clinical and musculoskeletal ultrasound features. To compare 15-PGDH concentrations among patients with knee osteoarthritis, and ankylosing spondylitis with knee effusion To evaluate whether serum 15-PGDH reflects intra-articular 15-PGDH concentrations. Analysis the level of 15-PGDH in relation to disease activity and severity of both diseases.

Inclusion Criteria: * Inclusion criteria * Adults aged 18 years or older. AxSpA group Patients diagnosed as ankylosing spondylitis according to ASAS criteria . R OA group * Adults with osteoarthritis Diagnosed according to the American College of Rheumatology classification criteria.\[8\]R ACR Clinical classification criteria for Osteoarthritis of the hip ACR Clinical classification criteria for Osteoarthritis of the knee ACR Clinical classification criteria for Osteoarthritis of the hand Control group Age- and sex-matched healthy controls. Exclusion Criteria: * • Other inflammatory arthritides such as psoriatic arthritis, gout, or SLE-related arthritis. * Septic arthritis or suspected joint infection. * Recent intra-articular steroid injection if it may influence biomarker results. * Any major systemic illness likely to affect biomarker measurement or ultrasound interpretation. * Patients received certain drugs as non-steroidal anti-inflammatory drugs (NSAIDs)