Assessment of Peridontal Inflamatuar Markers in MIH

The goal of this observational study is to investigate periodontal inflammatory mediators in systemically healthy children aged 8-13 years with and without molar-incisor hypomineralization (MIH) who require initial periodontal treatment due to gingivitis. The main questions it aims to answer are: Are the levels of periodontal inflammatory mediators in gingival crevicular fluid different between children with MIH and those without MIH? Is the presence and severity of MIH associated with clinical periodontal parameters and dentin hypersensitivity? Researchers will compare children with MIH to children without MIH to determine whether MIH is associated with changes in periodontal inflammatory mediator levels and periodontal clinical findings.

Inclusion Criteria: * Systemically healthy children aged 8-13 years (both sexes). * Children who demonstrate adequate cooperation for dental treatment and study procedures (Frankl Behavior Rating Scale score ≥3). * Study group: Children with molar-incisor hypomineralization (MIH) affecting at least three permanent first molars and requiring initial periodontal treatment for gingivitis. * Control group: Children without molar-incisor hypomineralization who require initial periodontal treatment for gingivitis. * Presence of gingivitis requiring initial periodontal therapy. Exclusion Criteria: * Use of antibiotics within the previous 1 month. * Radiographic evidence of periodontal destruction. * History of periodontal treatment within the previous 3 months. * Presence of systemic, chronic, or metabolic diseases. * Ongoing active orthodontic treatment. * Developmental enamel defects other than molar-incisor hypomineralization. * History of premature birth.