Early Signals of the Transition From Immune Quiescence to Activation in the Liver Allograft Microenvironment and in the Circulation

This is a prospective multi-center, longitudinal study to determine efficacy of 50 percent Immunosuppression (IS) reduction. One hundred fully eligible participants will reduce IS by 50 percent in two steps. Liver tests will be checked every 0.5 months through month 4, once a month through month 12, and every other month through month 18. Liver transplant (LTx) center visits will take place at screening, months 6, 12 and 18 after initiating IS dose reduction. A protocol driven liver biopsy to adjudicate the endpoint will be performed at 18 months. The duration of the study from time of starting IS dose reduction to the primary endpoint assessment is 18 months. The primary objective is to assess the efficacy of 50 percent IS dose reduction in children with Liver transplants (LTxs)

Inclusion Criteria: 1. Participant and parent or guardian must be able to understand and provide informed assent and consent, respectively 2. Recipient of a living or deceased donor Liver transplant (LTx) at \ 3 years but \ 6 months with baseline 12-hour trough levels \=50 percent dose increase in tacrolimus within 12 months of enrollment 5. Discontinued a second Immunosuppression (IS) agent within 12 months of enrollment 6. Systemic illness requiring chronic or recurrent use of IS for which there is a risk of reactivation if tacrolimus is reduced 7. Use of medication to treat systemic conditions which in the judgement of the investigator could influence results of the study 8. Active or chronic infection requiring treatment 9. Inability or unwillingness to comply with the study protocol 10. Use of investigational drug within 4 weeks (or 5 half-lives of investigational drug, whichever is longer) of enrollment 11. Has any condition that, in the opinion of the investigator, will interfere with safe participation in the trial