Orthodontic Treatment on Oral Microbiota and Salivary Proteins

Study on the changes in oral microbiota during orthodontic treatment in patients aged 8-18 years. Orthodontic patients treated at Stomatology Hospital of Xi'an Jiaotong University from March 2025 to March 2027 will be recruited. Prior to orthodontic treatment, routine preoperative examinations will be conducted, informed consent forms will be signed, baseline oral conditions will be recorded, and oral and psychological-related questionnaires will be completed. Patients will be informed of the need for regular oral hygiene check-ups, caries risk assessments will be performed, and plaque, saliva, and intraoral photographs will be collected. During orthodontic treatment, plaque samples will be collected every 3 months, and saliva samples will be collected every 6 months until the treatment is completed to evaluate the changes in oral microbiota throughout the orthodontic process.

Inclusion Criteria: * No carries and White spot * No missing teeth * No history of oral treatment * No malocclusion * No systemic diseases Exclusion Criteria: * Antibiotic use in the past three months * A history of long-term medication use * A history of dental fillings * Unable to attend regular follow-ups