Whole Genome Sequencing (WGS) on IVF Embryos and Individual Patients

This research project will seek to utilize developments in whole genome sequencing of preimplantation genetic diagnosis embryos to investigate the effect paternal age has on the de novo mutation rate of IVF embryos.The study will whole genome sequence DNA obtained from biopsied embryos from infertile women to acquire the mutational burden status. This will enable ascertainment of the de novo mutation rate in addition to potential mutational hotspots.

Inclusion Criteria:Couples undergoing IVF in which the male is 40 or olderConsanguineous couplesPatients with a known genetic mutationExclusion Criteria:Female patients with low ovarian reserve (< 10 follicles or FSH>10, AMH <1).