DiEtary Sodium Intake Effects on Ertugliflozin-induced Changes in GFR, reNal Oxygenation and Systemic Hemodynamics: the DESIGN Study

SGLT2 inhibitors such as ertugliflozin improve blood pressure and kidney outcomes in people living with diabetes through incompletely understood mechanisms, however, not all patients treated with SGLT2 inhibition have improved outcomes. Changes in kidney sodium handling is among the mechanisms by which SGLT2 inhibition may reduce blood pressure and drive beneficial kidney outcomes. This process is heavily dependent on daily sodium intake by patients receiving SGLT2 inhibitor treatment. In this study, the effect of daily sodium intake on SGLT2-inhibitor induced physiological effect is studied, including blood pressure regulation and kidney physiology.

Inclusion Criteria:Adults with previously diagnosed T2DM according to American Diabetes Association (ADA) criteriaHbA1c 6.5-10%Age 35-80 years of ageOverweight or obese with BMI: >25 kg/m2We will make every effort to enrol participants of all races/ethnicities."Both sexes (females must be post-menopausal; no menses >1 year; in case of doubt, Follicle-Stimulating Hormone (FSH) will be determined with cut-off defined as >31 U/L)Ability to provide signed and dated, written informed consent prior to any study proceduresEstimated GFR 60-90 ml/min/1.73m2 by CKD-EPI matching the eGFR range of most participants in VERTIS-CVSodium intake at baseline < 200 mmol/dayUACR < 30 mg/mmolAll participants need to be on a stable dose of Diabetes medication, including Metformin, SU, insulinAll participants need to be on a stable dose of RAS inhibitionExclusion Criteria:History of unstable or rapidly progressing renal diseaseEstimated GFR <60 mL/min/1.73m2 or eGFR > 90 mL/min/1.73m2 determined by CKD-EPIUACR > 30 mg/mmolCurrent/chronic use of the following medication: SGLT2 inhibitors, TZD, GLP-1RA, glucocorticoids, immune suppressants, antimicrobial agents, chemotherapeutics, antipsychotics, tricyclic antidepressants (TCAs) and monoamine oxidase inhibitors (MAOIs). Subjects on diuretics will only be excluded when these drugs cannot be stopped for the duration of the study.Chronic use of non-steroidal anti-inflammatory drugs (NSAIDs) will not be allowed, unless used as incidental medication (1-2 tablets) for non-chronic indications (i.e. sports injury, headache or back ache). However, no such drug can be taken within a timeframe of 2 weeks prior to renal testingHistory of diabetic ketoacidosis (DKA) requiring medical intervention (e.g. emergency room visit and/or hospitalization) within 1 month prior to the Screening visit.Current urinary tract infection and active nephritisRecent (<6 months) history of cardiovascular disease, including:Acute coronary syndromeChronic heart failure (New York Heart Association grade II-IV)Stroke or transient ischemic neurologic disorderSevere hepatic insufficiency and/or significant abnormal liver function defined as aspartate aminotransferase (AST) >3x upper limit of normal (ULN) and/or alanine aminotransferase (ALT) >3x ULNHistory of or actual malignancy (except basal cell carcinoma)History of or actual severe mental diseaseSubstance abuse (alcohol: defined as >4 units/day)Allergy to any of the agents used in the studyIndividuals who are investigator site personnel, directly affiliated with the study, or are immediate (spouse, parent, child, or sibling, whether biological or legally adopted) family of investigator site personnel directly affiliated with the studyInability to understand the study protocol or give informed consent