Assessment of Urinary Uromodulin and the Corresponding Urinary Exosomal UMOD mRNA Gene Expression as a Potential Biomarker of Diabetic Nephropathy

Diabetes is a major health problem prolonged untreated diabetes cause kidney diseases that can leads to end stage renal disease (ESRD). There are several biomarkers to access the DN severity but there are conflicting regarding the specifity and sensitivity of these biomarkers. So it is important to identify novel biomarker for early detection DN and progression toward ESRD to minimize the prevelance of CKD in human population. Uromodulin is a tubular protein encoded by UMOD gene which expressed predominantly in the epithelial cells of thick ascending limb (TAL) of henel's loop. This study was conducted to evaluate the level of urinary uromodulin and its corresponding exosomal mRNA gene expression as a potentional marker for early detection of DN in type 2 diabetic patients

Inclusion Criteria:Type 2 diabetic patients with not no album in ur is, micoalbuminuria and macroalbuminuriaExclusion Criteria:History or existence of non diabetic or obstructive kidney disease, urinary tract infection, liver disease, autoimmune, inflammatory or neoplastic diseases will be excluded from the study.