Individual Variability of Experimental Gingivitis Response

The study aims to investigate the genetic cause of the variability between individuals seen in the development of the gum disease, gingivitis. This will be carried out through a 3 week programme where all oral hygiene is ceased, allowing 'experimental gingivitis' to develop, followed by a period of recovery when tooth cleaning is restored. Clinical assessments and biological samples will be taken during the course of the study for further analysis.

Inclusion Criteria: * Age 18 to 35 * Caucasian * No history of periodontitis/periodontal treatment (based on self-reported history) * Medical history clear of cardiovascular disease, diabetes, cerebrovascular disease, kidney or liver disease, cancer, HIV, Tuberculosis and Hepatitis C (self-reported) * Never smoker, e-cigarettes or nicotine gum use (self-reported). Individuals who rarely socially smoked more than 5 years ago would be permitted to participate. * Minimum of 24 teeth present * Be willing and competent (verbally and cognitively) to give written informed consent and complete a medical history form * Be willing and physically able to carry out all study procedures * Not have had a scale and prophylaxis in the previous month or have one scheduled prior to the end of the study * Participant in good periodontal health, based on: 1. absence of interproximal probing pocket depths (PPD) and clinical attachment level (CAL) \> 3mm (with the exception of distal surfaces of third molars in the presence of impacted third molars) at the screening visit. 2. bleeding on probing (BOP) \< 30% at the Screening visit and BOP \