Individual Variability of Experimental Gingivitis Response

The study aims to investigate the genetic cause of the variability between individuals seen in the development of the gum disease, gingivitis. This will be carried out through a 3 week programme where all oral hygiene is ceased, allowing 'experimental gingivitis' to develop, followed by a period of recovery when tooth cleaning is restored. Clinical assessments and biological samples will be taken during the course of the study for further analysis.

Inclusion Criteria:Age 18 to 35CaucasianNo history of periodontitis/periodontal treatment (based on self-reported history)Medical history clear of cardiovascular disease, diabetes, cerebrovascular disease, kidney or liver disease, cancer, HIV, Tuberculosis and Hepatitis C (self-reported)Never smoker, e-cigarettes or nicotine gum use (self-reported). Individuals who rarely socially smoked more than 5 years ago would be permitted to participate.Minimum of 24 teeth presentBe willing and competent (verbally and cognitively) to give written informed consent and complete a medical history formBe willing and physically able to carry out all study proceduresNot have had a scale and prophylaxis in the previous month or have one scheduled prior to the end of the studyParticipant in good periodontal health, based on:absence of interproximal probing pocket depths (PPD) and clinical attachment level (CAL) > 3mm (with the exception of distal surfaces of third molars in the presence of impacted third molars) at the screening visit.bleeding on probing (BOP) < 30% at the Screening visit and BOP <10% at Day -7.Exclusion Criteria:Currently taking part in other clinical trialsPregnant or breastfeeding womenTaking medications including systemic anti-inflammatory medication within 3 months of the study (NSAIDs, no more than once per week and not in the week before sample collection, is permitted)Systemic antibiotic intake within 6 monthsPsychiatric conditions affecting participation in the studyThe participant is also a member of staff who is part of the study teamCurrent orthodontic treatmentRegular consumption of alcohol exceeding the government recommended levelsDenture wearer/presence of dental implants/bridgesHave oral piercingsObvious signs of untreated cariesTaking dietary supplements (e.g. multivitamins; antioxidants; fish oils)