Impact of Time Restricted Feeding on Daily Protein Requirements

The investigators aim to assess 24-hour protein kinetics at two different levels of protein intake: 0.8 (RDA) and 1.6g/kg/day (2RDA) during a normal dietary (TRAD) and a time restricted feeding (TRF) pattern in older adults.

Inclusion Criteria:Men and women ages 50-75 years.BMI 20-30 kg/m2 inclusive.Capable of providing informed consent.COVID-19 negative and/or asymptomaticExclusion Criteria:Subject who does not/will not eat animal protein sources.Body mass index <20 or >30.Hemoglobin <10g/dL at screening.Platelets <150,000/uL at screening.History of diabetes.History of chemotherapy or radiation therapy for cancer in the 6 months prior to enrollment.History of gastrointestinal bypass/reduction surgery.History of a chronic inflammatory disease (e.g. what?)Currently receiving androgen (e.g., testosterone) or anabolic (e.g., GH, IGF-I) therapy.Currently using prescription blood thinning medications.Currently using corticosteroid medications.Unable or unwilling to suspend aspirin use for 7 days prior to Visit 3.Unwilling to avoid using protein or amino-acid supplements during participation.Subjects who are unwilling to fast overnight.Participating in >200 minutes/week of vigorous exercise and/or >4 days/week of resistance training.