Platelet Enzymatically Oxidized Phospholipids (eoxPL) Characterisation in a Healthy Cohort on and Off Aspirin

Healthy volunteers (n = 28) were recruited from the workplace (14 male, 14 female; age range 20-50 years). Ethical approval, which included informed consent, was from Cardiff University, Schools of Medicine and Dentistry Research Ethics Committee (SMREC16/02). Following a 2-week period free from non-steroidal anti-inflammatory drugs (NSAIDs), peripheral blood was obtained and platelets isolated. Participants were commenced on aspirin 75 mg once daily for seven days and then provided a repeat blood sample. Following a 2-month period, participants were invited to repeat samples pre- and post-aspirin, and again a third time 2 months later.

Inclusion Criteria:two-week washout period from any non-steroidal anti-inflammatory drugs (NSAIDs) use.Ability to take aspirin 75 mg once daily for seven days and sampled thereafter, before stopping the aspirin.Willingness to repeat this process after two months and four months of the initial sampling date.Exclusion Criteria:* Recent NSAID use