Relationship Between Sperm Head Vacuoles and Sperm DNA Alterations in Infertile Men

In men presenting sperm alterations, the selection of genetically undamaged spermatozoa need to be improved in order to increase the success of assisted reproduction treatments. The aim of this study is to determine whether the presence of sperm head vacuoles is associated with sperm DNA alterations.

Inclusion Criteria: * men * 18 years old or more * affiliated to social security * with isolated teratozoospermia or oligo-astheno-teratozoospermia according to the WHO guidelines (2010): * sperm concentration \< 15 million per ml or sperm number \< 39 million per ejaculate * progressive motility \< 35% * morphologically normal forms \< 50% according to the modified David's classification (Auger et al., 2001 * mobile spermatozoa selected by density gradient centrifugation \> 1 million, in previous spermograms * with normal constitutional karyotypes (46, XY) * signed an informed consent Exclusion Criteria: * with abnormal constitutional karyotypes * with a major alteration of sperm parameters, with mobile spermatozoa selected by density gradient centrifugation \< 1 million * under tutorship or guardianship by court order